Development of rapid targeted and random protocols for MinION long reads sequencing in conjunction with bioinformatic pipelines for Senecavirus A, Leonardo Cardia Caserta, Jeremy Volkening, Beate Crossley, Claudio Afonso, Diego G. Diel.

Development and evaluation of metagenome2Vec, a machine learning algorithm for sequencing-based pathogen detection., Sai Narayanan, Sathyanarayanan Aakur Narasimhan, Arun Kumar Bagavathi, Akhilesh Ramachandran.

Genetic and antigenic characterization of an expanding H3 Cluster IV-A influenza A virus clade in US swine visualized by Nextstrain, Megan Neveau, Michael Zeller, Carine K. Souza, Bryan S. Kaplan, Phillip Gauger, Amy L. Vincent, Tavis K. Anderson.

Development of an updated Porcine reproductive and respiratory syndrome virus Assay, Mazen Ismail, Angela Burrell, Rohan Shah, Michelle Swimley, Bob Qiu.

Exploration and utilization of ‘Flongles’ for fast, cost-efficient whole genome sequencing in veterinary diagnostics, Dan Rejmanek, Javier Asin, Anibal Armien Medianero, Francisco A. Uzal, Beate Crossley.

Targeted next-generation sequencing as a tool to identify meat contamination in pet foods, Jobin Kattoor, Jake Guag, Sarah M. Nemser, Rebecca P. Wilkes.

Over 1,000,000 tests: leveraging veterinary diagnostic expertise to implement a high-throughput SARS-CoV-2 testing laboratory, Melissa Laverack, Rebecca L. Tallmadge, Roopa Venugopalan, Daniel Sheehan, Craig Riecke, Scott Ross, Brittany D. Cronk, Zachary Dwyer, April Dunn, Michael Williams, Ann Muldoon, Esra Dogan, Colleen Lyon, Maria Lais Finnerty, Jacob Leeser, Lina Covaleda, Magnolia Ariza, Katherine Churchill, Eduardo Bueno, Roxanne Coffey, Joe Flint, XiuLin Zhang, Rahim Rustamov, Mia R Everts, Jennifer Powers, Kim Potter, François Elvinger, Elizabeth Plocharczyk, Diego G. Diel.

Development of a real-time PCR assay for detection and differentiation of Mycoplasma ovipneumoniae and a novel respiratory-associated Mycoplasma species in ovine and caprine respiratory specimens and comparison to conventional PCR, Lance Wade Noll, Margaret A. Highland, Vaughn Hamill, Wai Ning Tsui, Elizabeth Porter, Nanyan Lu, Tesfaalem Sebhatu, Susan Brown, David Herndon, Paige C. Grossman, Jianfa Bai.

A case of caprine abortion associated with multiple zoonotic pathogens, Yung-Yi C. Mosley, Lisa Whittington, Cynthia K. Watson, Michele D. Coarsey, Allen Aref Kalantari, Jill U. Johnson, Seth Stowers, Ingrid Fernandez-Marrero, Hemant K. Naikare.

High throughput diagnostic and screening tests for human COVID-19 at the Georgia Veterinary Diagnostic Laboratories: challenges and resolutions, Yung-Yi C. Mosley, Susan Sanchez, Lisa Whittington, Paula B. Bartlett, Amy McKinney, Hemant K. Naikare, Jesse Hostetter, Richard K. Harris, Deborah A. Blakey, Michelle Norris, Allen Aref Kalantari, Ravindra Kolhe, Shauna Corsaro, Jeff Duke.

Multiplexed PCR for detection of Streptococcus equi subsp. equi and Streptococcus equi subsp. zooepidemicus and differentiation of swine-associated highly pathogenic S. equi subsp. zooepidemicus,
Laura Bradner, Nubia Macedo, Eric R. Burrough, Orhan Sahin, Ganwu Li, Rodger Main, Panchan Sitthicharoenchai, Rachel Jean Derscheid, Maria Jose Clavijo, Karen M. Harmon.

Porcine Sapelovirus – correlation of histopathology and viral agent detection via chromogenic in situ hybridization, Kate Alucard, Jennifer Groeltz-Thrush, Mathias Afayoa, Rachel Jean Derscheid.

Development of a RT-LAMP assay for the detection of SARS-CoV-2 in companion animal samples at the University of Pennsylvania Ryan Veterinary Hospital, Eli Adzema Braun, Jaclyn Dietrich, Stephen Cole, Shelley Rankin.

Development and validation of a swine enteric coronavirus 4-plex real-time RT-PCR and investigation of virus detection frequency in US swine, Jinhui Zhu, Gaurav Rawal, Ethan Aljets, Wannarat Yim-im, Yong-Le Yang, Yao-Wei Huang, Karen M. Harmon, Phillip Gauger, Jianqiang Zhang.

VETSCAN IMAGYST object recognition deep-learning algorithm improvement for the detection of Ancylostoma spp. eggs in feces from dogs and cats, Yoko Nagamori, Ruth Hall Sedlak, Benjamin S. Larson, Richard Boyd Smith, Micheal Loenser, Cory Penn, Richard Goldstein.

Non-target RNA depletion strategy to improve sensitivity of next generation sequencing (NGS) for diagnosing RNA viruses in poultry, David Suarez, Joshua Parris, Henry Kariithi.

Comparison of CWD diagnostics in wild white-tailed deer with new commercially available options for both ELISA and RT-QuIC, Maria Pansulla, Cheryl Chang, Jan Yacabucci, John Armstrong, Erick Gagne, Michelle Lucey.

Loop-mediated isothermal amplification and DNA sequencing for the detection and genotyping of equine herpesvirus type 1 in equine nasal secretions, Nadia Saklou, Stephanie Michelle Todd, Brandy Burgess, Kevin Lahmers.

Genomic characterization of a bovine-like coronavirus detected in an odd-toed non ruminant tapir, Leyi Wang, Christian Savard, Chantale Provost, Olivier Ariel, Samuel Morin, Richard Fredrickson, Carl Gagnon, André Broes.

Time and temperature stability of Tritrichomonas foetus RNA in phosphate buffered saline as evaluated by a reverse transcription real-time PCR assay, Duan Sriyotee Loy, Renata Spuri Gomes, Bruce Brodersen, John Dustin Loy.

Molecular Diagnostics and Bioinformatics
Development of rapid targeted and random protocols for MinION long reads sequencing in conjunction with bioinformatic pipelines for Senecavirus A
Development and evaluation of metagenome2Vec, a machine learning algorithm for sequencing-based pathogen detection
Genetic and antigenic characterization of an expanding H3 Cluster IV-A influenza A virus clade in US swine visualized by Nextstrain
Development of an updated Porcine reproductive and respiratory syndrome virus Assay
Exploration and utilization of ‘Flongles’ for fast, cost-efficient whole genome sequencing in veterinary diagnostics,
argeted next-generation sequencing as a tool to identify meat contamination in pet foods
Over 1,000,000 tests: leveraging veterinary diagnostic expertise to implement a high-throughput SARS-CoV-2 testing laboratory
Development of a real-time PCR assay for detection and differentiation of Mycoplasma ovipneumoniae and a novel respiratory-associated Mycoplasma species in ovine and caprine respiratory specimens and comparison to conventional PCR
A case of caprine abortion associated with multiple zoonotic pathogens
High throughput diagnostic and screening tests for human COVID-19 at the Georgia Veterinary Diagnostic Laboratories: challenges and resolutions
Multiplexed PCR for detection of Streptococcus equi subsp. equi and Streptococcus equi subsp. zooepidemicus and differentiation of swine-associated highly pathogenic S. equi subsp. zooepidemicus
Porcine Sapelovirus – correlation of histopathology and viral agent detection via chromogenic in situ hybridization
Development of a RT-LAMP assay for the detection of SARS-CoV-2 in companion animal samples at the University of Pennsylvania Ryan Veterinary Hospital
Development and validation of a swine enteric coronavirus 4-plex real-time RT-PCR and investigation of virus detection frequency in US swine
VETSCAN IMAGYST object recognition deep-learning algorithm improvement for the detection of Ancylostoma spp. eggs in feces from dogs and cats
Non-target RNA depletion strategy to improve sensitivity of next generation sequencing (NGS) for diagnosing RNA viruses in poultry,
Comparison of CWD diagnostics in wild white-tailed deer with new commercially available options for both ELISA and RT-QuIC
Loop-mediated isothermal amplification and DNA sequencing for the detection and genotyping of equine herpesvirus type 1 in equine nasal secretions,
Genomic characterization of a bovine-like coronavirus detected in an odd-toed non ruminant tapir
Time and temperature stability of Tritrichomonas foetus RNA in phosphate buffered saline as evaluated by a reverse transcription real-time PCR assay

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